The Molecular Biomethods Handbook, 2nd Edition offers an updated collection of techniques in biosciences, focusing on nucleic acid, protein, and cell-based methodologies. It includes practical procedures and applications for gene expression profiling, protein engineering, and more, serving as a vital reference for molecular biology researchers.
A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.