Dieses Buch umfasst alle modernen Methoden, die heute in der Biochemie und Molekularbiologie eingesetzt werden. Im ersten Teil des Werkes, das sich in der ersten Auflage rasch als Standardwerk etabliert hat, werden die Grundlagen der gängigen instrumentalanalytischen Techniken gelegt - etwa Chromatographie, Elektrophorese und Massenspektrometrie -, die später auf andere Klassen von Biomolekülen, die Kohlenhydrate, Lipide und Nucleinsäuren, angewendet werden. Breiter Raum ist den Verfahren der systematischen Funktionsanalytik gewidmet.
Analytical Methods and Concepts in Biochemistry and Molecular Biology
1134 Seiten
40 Lesestunden
Analytical methods are crucial tools in modern biosciences. This book offers a comprehensive introduction to these methods, detailing their physical and chemical foundations, along with a discussion of their strengths and weaknesses. It encompasses all major techniques for analyzing biological macromolecules, including proteins, carbohydrates, lipids, and nucleic acids. Frequent cross-references highlight the connections between different techniques, providing a broad overview of the subject and aiding readers in selecting the most suitable method for specific bioanalytical challenges. This resource is invaluable for students and researchers in designing and evaluating experimental research, while also presenting new material for seasoned experimentalists. Key techniques discussed include purification and determination of proteins, enzymatic activity measurement, microcalorimetry, immunoassays, affinity chromatography, cross-linking, light and electron microscopy, chromatographic and electrophoretic techniques, mass spectrometry, biosensors, NMR and EPR of biomolecules, carbohydrate and lipid analysis, nucleic acid isolation, DNA hybridization, polymerase chain reaction techniques, and various analyses related to protein-nucleic acid interactions, proteomics, metabolomics, and chemical biology.
