Harry Walter Bücher

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features* Use of affinity partitioning for enzyme purification* Separation and study of cells* Isolation of plasma membranes* Large scale biotechnical use