Yiyang Dong Bücher

An essential guide focusing on method developments and applications in aptamers, this resource explores the growing field of aptamer-based systems for various analytical and medical uses. It introduces aptamers, outlines common synthesis protocols, and details optimization strategies for achieving higher affinities to target molecules. Contributions from noted experts provide an in-depth review of aptamer-target interactions and immobilization techniques, discussing method advancements across relevant applications. The book presents various schemes for efficiently immobilizing aptamers on substrates and summarizes characterization methods for aptamer-ligand complexes. It covers aptamer-based analytical methods, including colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow, and non-labeling techniques. Additionally, it reflects on state-of-the-art and emerging applications of aptamer-based methods. This important resource offers a guide to highly specific and sensitive molecular recognition, with affinities comparable to antibodies but at a lower production cost. It discusses analytical method developments and improvements while providing a comprehensive overview of relevant application areas. Written for analytical chemists, biochemists, and researchers, this book adopts a methodological perspective on aptamer generation and modification, emphasizing method developments for practical applications.

Clear, comprehensive, and state-of-the-art, this groundbreaking book delves into the emerging technology of direct analysis in real time mass spectrometry, authored by a noted expert in the field. It reviews the background and recent developments in DART-MS, a technique invented in 2005 that addresses various analytical challenges across multiple environments, including food science, forensics, and clinical analysis. The text introduces the technology's history and theoretical background, particularly the ionization mechanism. It includes chapters on sampling and the coupling of DART to different mass spectrometers, followed by an extensive discussion of diverse applications. Notably, DART requires no laborious sample preparation, as ionization occurs directly on the sample surface, making it particularly appealing for forensic and food science applications. This vital text sets the standard for this important ionization technique, thoroughly discussing instrumentation, applications, and addressing challenges and disadvantages. It serves as an essential resource for academics, analytical chemists, pharmaceutical chemists, clinical chemists, forensic scientists, and others seeking to understand the latest advancements in the field.