Focusing on the interdisciplinary field of nanobiosensing, this book delves into the latest research at the intersection of chemistry, biology, and materials science. It explores innovative detection principles and device engineering methods while highlighting key nanomaterials such as carbon nanotubes and quantum dots. The text also covers various sensing mechanisms, including electrochemical detection, fluorescence, and antibody-antigen interactions, providing a comprehensive overview of the advancements in biosensing and bioanalysis.
This book summarizes the biology of microRNA (miRNA) in various pathological processes, highlighting their potential in diagnostics, prognostics, and as novel drug targets. It covers traditional detection techniques such as standard PCR, Northern blotting, microarrays, and cloning methods. Additionally, it explores emerging strategies for miRNA detection and quantification, emphasizing new molecular biological techniques, locked nucleic acid (LNA) modified probes, and nanotechnology-based approaches. The latest advances in clinical miRNA detection methods in living cells, circulating blood, and tissues are discussed, including in situ hybridization (ISH) and molecular imaging techniques, which help elucidate miRNA biogenesis and function in vivo. The book also examines the advantages and drawbacks of various detection techniques in this rapidly evolving field, addressing challenges and promising new directions. It serves as a valuable resource for analytical chemists, biologists, and physicians engaged in miRNA research. The authors, Dr. Xueji Zhang and Dr. Haifeng Dong, are Professors at the School of Chemistry & Biological Engineering at the University of Science & Technology Beijing, while Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School.
This book broadly reviews the modem techniques and significant applications of chemical sensors and biosensors. Chapters are written by experts in the field – including Professor Joseph Wang, the most cited scientist in the world and renowned expert on sensor science who is also co-editor. Each chapter provides technical details beyond the level found in typical journal articles, and explores the application of chemical sensors and biosensors to a significant problem in biomedical science, also providing a prospectus for the future.This book compiles the expert knowledge of many specialists in the construction and use of chemical sensors and biosensors including nitric oxide sensors, glucose sensors, DNA sensors, hydrogen sulfide sensors, oxygen sensors, superoxide sensors, immuno sensors, lab on chip, implatable microsensors, et al. Emphasis is laid on practical problems, ranging from chemical application to biomedical monitoring and from in vitro to in vivo, from single cell to animal to human measurement. This provides the unique opportunity of exchanging and combining the expertise of otherwise apparently unrelated disciplines of chemistry, biological engineering, and electronic engineering, medical, physiological.
