Rolf Theodor Borlinghaus Bücher

Light sheet microscopy is distinguished by its unique observation and illumination angles, offering enhanced clarity by illuminating only a small part of the specimen. The book explores the historical principles and technical concepts behind this innovative microscopy technique, while also presenting its current applications. It emphasizes the careful selection of optical elements that contribute to producing sharp images without blurring, making it a significant advancement over traditional light microscopy.

This book offers a comprehensive introduction to confocal microscopy – with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an outline of fluorescence and its corresponding implications for microscopy. The following excursus on the confocal beam paths includes implementation of acousto-optical devices and modern sensor techniques. Complex relationships are explained in a comprehensible manner, supported by many graphical figures. Discussing the principles of magnifying optics and the technical fundamentals and modes of operation of modern laser scanning microscopes, it is a valuable resource for student and lab technicians as well as faculty members.